ABSTRACT
Conclusions:
RAA and RV-FAC are easily obtained and should be evaluated in potential LVAD patients. Risk assessment systems should also take into account the presence of ascites and low prealbumin levels which are not currently incorporated into any risk models. Validation of the relative importance of all of these parameters requires further investigation.
Methods and Results:
Forty-two patients were implanted with LVADs (32 HeartWare® ventricular assist device and 10 Thoratec® HeartMate II) between March 2013 and April 2014 at the Ege University Medical School, Izmir, Turkey. Baseline clinical, demographic, and laboratory information were measured and patients prospectively fallowed after surgery. Endpoint was defined RVF development for patients. Before surgery, hemodynamic parameters, electrocardiographic findings, standard echocardiographic measurements, and medications were recorded. Multivariate regression analysis showed that the presence of ascites, prealbumin <14 mg/dl, bilirubin >1.5 mg/dl, RV diameter >3.2 cm, RV-fractional area change (FAC) <24%, right atrial area (RAA) >28 cm, 2 and RV-myocardial performance index >0.35 were the strongest predictors of RVF after LVAD implantation.
Background:
Right ventricular (RV) failure is a serious adverse event for patients with left ventricular assist devices (LVADs). Here, we seek to identify the risk factors which may predict the development of RV failure (RVF).
Introduction
Right ventricular failure (RVF) is an important cause of the morbidity and mortality that develops in patients with left ventricular assist devices (LVADs). RVF prolongs stay in the intensive care unit, increases 30-day mortality, and decreases the transplantation bridging rates.[1],[2] INTERMACS has defined RVF as a condition leading to low cardiac output, high central venous pressure (CVP), low left ventricle filling pressure, and requires inotrope infusions for longer than 14 days or ECMO or right ventricular assist devices (RVAD).[3]
The incidence of RVF after LVAD implantation varies from 6 to 44% according to recent studies. This large variability is due to differences in definitions of RVF and the patient populations of the studies.[4] Some authors defined RVF only as a condition of need RVAD,[5],[6],[7] and some others especially INTERMACS defined RVF as a condition leading to low cardiac output, high CVP, low left ventricle filling pressure, and requires inotrope infusions or need for RVAD. Based on riquirement of inotrope time, RVF has been classified by INTERMACS as mild (within 7 days), modarate (7-14 days) and severe (>14 days or need for RVAD). The complex pathophysiology of RVF makes it difficult to predict the patients who are susceptible to developing this condition. The structure of the right ventricular (RV) myocardium and pathologies of the pulmonary vascular bed may contribute to the formation of RVF. Perioperative factors such as myocardial stunning, ischemia, infarction, and arrhythmias can also lead to RVF. Finally, with mechanical circulatory support, the sudden septal displacement which occurs during the emptying of the left ventricle as well as the increase in preload may contribute to the deterioration of right ventricle.[1],[8] The purpose of this study was to identify the clinical, laboratory, hemodynamic, and echocardiographic risk factors that may predict the development of RVF in end-stage heart failure patients requiring LVADs.
Materials and Methods
Patient population
Fifty patients were initially enrolled in this prospective study. Bleeding is a known risk factor for RVF and so patients who underwent revision due to bleeding after LVAD implantation were excluded. The forty-two remaining patients were implanted with LVADs between March 2013 and April 2014. The HeartWare® ventricular assist device (HVAD, HeartWare Inc., Framingham, MA) was implanted in 32 (76%) of the patients, while the HeartMate II (HM2, Thoratec Corp., Pleasanton, CA) was implanted in 10 (24%) patients.
In this study, RVF was defined as the need for inotropes for more than 14 days and/or need for RVAD implantation. To identify the risk factors for RVF after LVAD implantation, baseline values were collected for the patients' clinical, demographic, and laboratory information. Immediately before surgery, patient hemodynamic parameters, electrocardiographic findings, and medications were also recorded. Finally, the Michigan RV risk score (vasopressor need: 4 points, aspartate aminotransferase [AST] >80:2 points, total bilirubin >2:2.5 points, creatinine >2.3:3 points) and Lietz–Miller risk score (thrombocyte <148000:7 points, albumin <3.3:5 points, INR >1.1:4 points, vasodilator treatment: 4 points, mean pulmonary artery pressure [PAP] <25 mmHg: 3 points, AST >45:2 points, Htc <34%:2 points, BUN >51:2 points, no need for IV inotropes: 2 points) were calculated for all patients. For the analysis, patients were divided into ischemic and nonischemic groups according to the etiology of their heart failure and also were categorized into INTERMACS groups (1/2, 3/4, and 5–7).
Echocardiographic measurements
Before surgery, all patients underwent transthoracic echocardiography to obtain standard echocardiographic measurements (VIVID 7 PRO, GE, M4S probe, 1.5–4.3 MHz). The RV fractional area change (RV-FAC), the right atrial area (RAA), RV outflow tract (RVOT), the proximal (RVOT1, diastole and systole) and distal (RVOT2) diameters, the RVOT fractional shortening (RVOT-FS), the RV diameters (RVD1, RVD2, RVD3), the tricuspid regurgitation velocity, and the systolic PAP values were calculated according to the guidelines of the American Society of Electrocardiography. The ratio of the RV diameter to the left ventricular diameter (LV diameter) was measured with two-dimensional measurements in apical and parasternal slices separately (RV/LV ratio apical, RV/LV ratio parasternal). The RV myocardial performance index (MRI) was calculated in all cases using the pulsed wave Doppler technique.[9] The parasternal short-axis slice was measured at the aortic valve level and the anterior wall of the RVOT. The systolic displacement of the RVOT was measured using the M-mod technique (RVOT-SE).[10]
Statistical analysis
All variables were expressed as percentages or mean ± standard deviation. All continuous variables were compared with the Mann–Whitney U test and categorical variables were compared with the Chi-square or Fisher's exact test. Logistic regression analyses were performed for the variables that were significantly associated with RVF. ROC curve analyses were performed to identify the cutoff values, diagnostic sensitivity, and specificity of the variables significantly associated with the development of RVF. P < 0.05 was considered statistically significant and all analyses were performed using SPSS version 18.0 (SPSS Inc., Chicago, USA).
Ethical statement
This scientific research was initiated with the desicion of Ege Univercity Faculty of Medicine Clinical Research Board dated 19/03/2013 and numbered 13-2.2/1.
Results
RVF occurred in 7 (16.7%) patients during follow-up. One patient required an RVAD and the six others required more than 14 days of inotropes. The preoperative baseline characteristics and comorbidity conditions of the patients in both the groups are shown in [Table 1] with no significant differences observed between RVF and non-RVF patients. Ischemic etiology was noted in 57% (n = 24) of the patients and 43% (n = 18) were nonischemic, with no significant association between etiology and RVF. There was, however, a significant association between a low INTERMACS class (class 1 and 2) and the development of RVF (P = 0.017).
The hemodynamic parameters in [Table 2] show patients who developed RVF had significantly lower systolic and diastolic blood pressures. Although RVF occurred more often in patients who required inotropes the difference was not statistically significant (P = 0.063). In addition, ascites was identified in seven patients before surgery and 4 (57%) of those patients developed RVF. Despite the small sample size, there was a significant association between the presence of ascites and RVF (P = 0.009). The analysis of the preoperative medication data showed that there were no significant differences in the patients with and without RVF for all medications except for angiotensin-converting enzyme inhibitors (ACE-I). All 15 patients using ACE-I did not develop RVF which was significantly different from the 7 of 27 (25.9%) of patients not using ACE-I who later developed RVF (P = 0.038).
Preoperative right heart catheterization (RHC) showed no significant differences between the patients with and without RVF except in the case of mean right atrial pressure (RAP). Patients who developed RVF had significantly higher RAP (15 ± 6 vs. 9 ± 4 mmHg, P = 0.039). There were generally no differences in the biochemical laboratory results [Table 3]. Patients with RVF tended to have lower leukocytes and albumin levels but the differences were not statistically significant. The mean prealbumin levels were significantly lower for the patients with RVF (P = 0.003). RVF patients also had significantly higher bilirubin and NT-proBNP levels (P = 0.008 and P = 0.041, respectively).
The results of current RV risk assessment methods are in [Table 4]. The Michigan RV Risk score showed a significant association between a high-risk assessment and eventual development of RVF (P = 0.02). However for this cohort, the Lietz–Miller Risk Assessment was not successful in identifying which patients were at high risk and eventually developed RVF (P = 0.203). Echocardiographic measurements [Table 5] showed that RVF was associated with an overall larger right ventricle as RV diameter, RAA, tricuspid annular diameter, and the ratio of the right to left ventricle were all significantly higher for patients with RVF. As expected, patients with RVF also showed many signs of deteriorated heart condition as they had significantly worse ejection fraction, tricuspid regurgitation, RV-FAC, tricuspid annular plane systolic excursion (TAPSE), and RV- MRI. Despite the changes in the RV, often patients with and without RVF did not show any significant differences in their left ventricle echocardiographic measurements.
Results of the multiple logistic regression analyses of risk factors for RVF are in [Table 6]. The presence of ascites, prealbumin <14 mg/dl, total bilirubin >1.5 mg/dl, RV diameter >3.2 cm, RV-FAC <24%, RAA >28 cm2, and RV-MPI >0.35 were the strongest predictors of RVF. The ROC curves in [Figure 1] show the specificity and sensitivity of pre-albumin, RV-FAC, RAA, and RV-MPI for predicting the development of RVF.
Discussion
The results of our study show that baseline characteristics or comorbidity conditions were not significantly associated with the development of RVF in patients undergoing LVAD placement. Previous studies have suggested that RVF develops more frequently in female patients and those with nonischemic etiology, smaller BSAs, or advanced age.[11],[12],[13] However, in a study of 197 patients, Matthews et al.[14] reported that there were no significant differences in those baseline characteristics similar to the results of this analysis. Furthermore, a large meta-analysis published in last years reported that female gender is an independent predictor of early RVF.[15] A low INTERMACS class has been previously reported as a risk factor for RVF,[16] which is understandable since a lower classification indicates the patient has poor cardiac reserves and systemic circulation. In this cohort, there was also a significant association between low (1/2) INTERMACS class and RVF. In contrast, patients with INTERMACS class 3/4 had a relatively low risk of developing RVF. There was also no significant difference in RVF between groups with and without inotrope use. This may suggest earlier LVAD implantation, before the patient status deteriorates to INTERMACS class 1/2, has the potential to improve morbidity and mortality.
The association between ascites and RVF has not been analyzed previously and the presence of ascites is not considered in any current RVF risk score system. As there was a significant association between the presence of ascites and RVF, it is recommended to include this condition, which should be simple to evaluate in a new risk score system. Univariate analysis showed RVF developed significantly less often in patients using ACE-I. However, in the multiple logistic regression analysis, it was determined that the difference was due to the patients' INTERMACS class and ACE-I use was not an independent significant risk factor of RVF.
Postoperative right heart catheterization revealed a statistically significant association between high RAP and RVF, which has similarly been reported in previous studies.[14],[16],[17] In this cohort, the other catheter measurements were not associated with the development of RVF as pulmonary artery pressures were not significantly different between the two groups. However, Fukamachi et al.[12] previously reported that a low mean PAP values can be a risk factor for RVF. Alnsasra et al.[18] reported that elevated diastolic pulmonary gradient is associated with RVF. Dandel et al. reported in their study including 475 patients that elevated CVP is an independent predictor of postoperative RVF.[19] Wang et al.[5] also reported that elevated CVP/pulmonary capillary wedge pressure ratio levels have a relation between postoperative RVF. Because of the many underlying mechanisms of RVF development, differences in the patients could lead to these conflicting results.
Although laboratory measurements such as AST, bilirubin, and creatinine can indicate end organ damage, changes in these markers are not specific to RVF. In this study, there was no correlation between most of the laboratory measurements and the development of RVF [Table 3]. However, prealbumin was significantly lower in patients with RVF and prealbumin values of <14 mg/dl predicted RVF with 80% specificity and 71% sensitivity. Prealbumin could serve as a sensitive and early indicator for RV dysfunction since it is a negative acute phase reactant and has a short half-life as previously described.[20] RVF patients also had significantly higher levels of bilirubin which concurs with other reports.[21],[22] Previous studies have identified associations between RVF and increased AST, creatinine, and NT-proBNP levels.[12],[13],[23]
As expected, preoperative echocardiographic data revealed that there was a significant increase in size and decrease in function of the RV in patients who later developed RVF. This is consistent with previous literature where there was increased right heart size based on RV/LV ratio and tricuspid annulus diameter in patients with RVF.[23],[24],[25],[26] Similarly, right heart function was diminished for those patients as measured by tricuspid regurgitation, TAPSE, RV-FAC, and RVOT-SE.[10],[16],[27],[28] While previous work has shown that RVOT-SE is predictive of decreased RV function, this study revealed a correlation between RVOT-SE and RVF development after LVAD implantation.
Study limitations
Limitation of this study are that patient data were collected from a single center and site-specific practices may have had an effect on the results. For example, at this institution, patients are often implanted with an LVAD earlier to prevent development of end organ damage. This is supported by the much lower percentage of INTERMACS class 1 and 2 patients receiving the device (19% of cohort) compared to other studies. Because of this earlier implantation practice, it is possible our results did not show significant associations between RVF and certain laboratory measures of end organ damage. In addition, the smaller sample size may have limited our statistical analysis. While the univariate analysis revealed a significant association between RVOT-SE and RVF, this significance was not shown with multiple logistic regression analyses. To better determine if variables such as RVOT-SE are risk factors, larger patient groups should be considered.
Conclusions
Several hemodynamic, biochemical laboratory, and echocardiographic measurements were significantly associated with the development of RVF in this prospective single-center study. Multivariate regression analysis showed that the presence of ascites, pre-albumin <14 mg/dl, bilirubin >1.5 mg/dl, RV diameter >3.2 cm, RV-FAC <24%, RAA >28 cm2, and RV-MPI >0.35 were the strongest predictors of RVF after LVAD implantation. These findings suggest that RAA and RV-FAC, which are easily measured with standard transthoracic echocardiography, should be evaluated in patients with plans for LVAD implantation. Similarly, risk assessment systems should take into account the presence of ascites and low prealbumin levels. Validation of the relative importance of all of these parameters requires further investigation. In this study, the risk for developing RVF was also significantly lower in INTERMACS class 3/4 patients compared to the class 1/2 cohort. This has influenced this institution's LVAD implantation strategies as we believe that earlier LVAD implantation might provide significant morbidity and mortality benefits.
Declaration of patient consent
The authors certify that they have obtained all appropriate patient consent forms. In the form, the patients have given their consent for images and other clinical information to be reported in the journal. The patients understand that their names and initials will not be published and due efforts will be made to conceal their identity, but anonymity cannot be guaranteed.
Acknowledgments
The authors acknowledge Mary V. Jacoski and Ming-Jay Chow, HeartWare Inc., for their assistance in the preparation of the manuscript.
Financial support and sponsorship
Nil.
Conflicts of interest
There are no conflicts of interest.